Application

Western Blotting: 1-10 µg/mL for affinity pure antibody using Chemiluminescence technique. An antibody made to the AQP2 peptide detected a major band at ~29 kDa in rat kidney cortex, and 35-47 kDa in inner and outer medulla (postranslational or glycosylation) (Nielsen et al. 1993; Deen et al. 1995). A similar pattern is seen in the human kidney (Sasaki, et al. 1994).

Immunohistochemistry: 2-10 µg/mL in paraformaldehyde fixed sections of tissues. AQP2 has been localized in apical membranes (Fushimi et al. 1993; Nielsen et al. 1993; Deen et al. 1995).

ELISA: 0.5-1.0 µg/mL using 1 µg/mL control peptide/well.

Optimal working dilutions must be determined by end user.

Detect Aquaporin 2 using this Anti-Aquaporin 2 Antibody validated for use in ELISA, IH & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 15 aa C-terminal peptide from rat AQP2. This peptide is predicted to be cytoplasmic.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin.Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

Specificity

Aquaporin 2 (AQP-CD/WCH-CD).

SPECIES REACTIVITY: The antibody has not yet been tested for cross-reactivity to other species. The 15 aa C-terminal peptide has 100% homology with mouse and 93% homology with human AQP2.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.